Mechanism of the separation process in paper chromatography
The solvent rises through the wick and the components are separated into concentric rings. In this technique a square or rectangular paper is used. Here the sample is applied to one of the corners and development is performed at a right angle to the direction of the first run. The discovery of paper chromatography in by Martin and Synge provided, for the first time, the means of surveying constituents of plants and for their separation and identification. There was an explosion of activity in this field after From Wikipedia, the free encyclopedia. This article needs additional citations for verification.
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Chromatographic Separation Methods
Retrieved Affinity chromatography Column chromatography Displacement chromatography Electrochromatography Gas chromatography High-performance liquid chromatography Capillary electrochromatography Ion chromatography Micellar electrokinetic chromatography Normal-phase chromatography Paper chromatography Reversed-phase chromatography Size-exclusion chromatography Thin-layer chromatography Two-dimensional chromatography.
Gas chromatography—mass spectrometry Liquid chromatography—mass spectrometry Pyrolysis—gas chromatography—mass spectrometry.
Distribution constant Freundlich equation Kovats retention index Retention factor Van Deemter equation. Category Commons Analytical Chemistry. Authority control NDL : Categories : Chromatography. Hidden categories: Articles needing additional references from February All articles needing additional references Wikipedia articles with NDL identifiers.
Producing a paper chromatogram
Namespaces Article Talk. Views Read Edit View history. In paper chromatography separations the component is distributed between the water held between the pores of filter paper and the liquid phase which travels along the filter paper….. As the name suggests the developing solvent moves in an upward direction. Sufficient quantity of mobile phase is poured into the development chamber. Sample and reference are spotted on the line drawn a few centimeters from the bottom edge of the paper suspended from a hook or clip at the top…..
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Paper chromatography makes use of paper which acts as a stationary phase. Paper essentially consists of cellulose fibers which are polymers having — OH functional groups sticking out of the polymer chains. These groups lead to retention and separation of surface absorbed molecules……. A solvent plays an important role in resolution of sample constituents on the filter paper.
For the purpose use can be made of a single pure solvent, undiluted solvent with water or a mixture of miscible solvents. The greater the distance between the resolved spots measured by Rf values better is the solvent mixture combination….. Samples can come either as liquids or solids. Liquids can be spotted without any pretreatment.
However, solid samples need to be dissolved in a suitable solvent as only liquids can be applied to the chromatography paper….. Footnotes Conflict of Interest: None declared. Selective enzyme purification by affinity chromatography. Porath J. From gel filtration to adsorptive size exclusion. J Protein Chem. Harris DC. Exploring chemical analysis. Preparative ion-exchange chromatography of proteins from dairy whey. J Chromatogr A. Introduction to organic laboratory techniques. Experimental organic chemistry:Principles and Practice.
Oxford:Blacwell Science; —5. Das M, Dasgupta D.
Pseudo-affinity column chromatography based rapid purification procedure for T7 RNA polymerase. Prep Biochem Biotechnol. Principles, High Resolution Methods, and Applications. Ion exchange chromatography. New York: Wiley; Amercham Biosciences. Ion Exchange chromatohgraphy, Principles and methods, Amercham Pharmacia. Biotech SE. Walls D, Loughran ST. Protein chromatography:Methods and protocols, methods in molecular biology.
Helmut D. Gel Chromatography, gel filtration, gel permeation, molecular sieves:a laboratory hand book. Springer-Verlag; Determann H. Gel chromatography gel filtration, gel permeation, molecular sieves:a laboratory handbook. Chapter 2. Materials and Methods. Wilchek M, Chaiken I.
Chromatography- Principle, Types and Applications
An overview of affinity chromatography in affinity chromatography—Methods and protocols. Humana Press. Firer MA. Efficient elution of functional proteins in affinity chromatography. J Biochem Biophys Methods. TLC plates as a convenient platform for solvent-free reactions. Chem Commun Camb ; 12 —1. Engel introduction to organic laboratory techniques. Amicon, Dye-ligand chromatography.
Applications method. Theory of matrix gel media, Amicon Division. Scopes RK.
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- Chromatography Limitations.
Use of differential dye-ligand chromatography with affinity elution for enzyme purificationketodeoxyphosphogluconate aldolase from Zymomonas mobilis. Anal Biochem. Cutler P. Methods in molecular biology, Dye-ligand affinity chromatography. Totowa, NJ: Humana Press; Mahn A, Asenjo JA.
Prediction of protein retention in hydrophobic interaction Chromatography. Biotechnol Adv.